盐酸大观霉素ph值：潮霉素BHygromycinB
盐酸大观霉素ph值：潮霉素BHygromycinBa) 3000 synchronized L1 larvae were transferred to seeded 6 cm NGM plates and immediately treated with hygromycin B. Images of control and treated plates were acquired 40 hours later. b) Survival assay on hygromycin B dilution. 3000 synchronised L1 larvae were plated on seeded 6 cm plates and immediately treated with hygromycin B at the specified final concentrations. The plates were scored for L4
潮霉素B(Hygromycin B)是由吸水链霉菌产生的抗生素。潮霉素B是一种氨基糖苷类抗生素，通过抑制蛋白质合成来杀死细菌，真菌和高等真核细胞。
溶解度SolubilityDMSO: 3.3 mg/ml PBS (pH 7.2): 3.3 mg/ml 溶于水
性状：白色固体粉末
储藏条件：Store desiccated at -20°C.
潮霉素B 的应用：
潮霉素B 通常用于研究蛋白质合成。潮霉素B 除抗细菌，真菌和高等真核细胞的功效外，潮霉素B 还是一种抗病毒剂，可在选择性进入因病毒感染而变成可渗透的细胞后抑制翻译。它在基因实验中用作标准选择抗生素，尤其是对于潮霉素抗性基因转化细胞的选择。
潮霉素B In vivo：
1.逐一添加每种溶剂：10％DMSO 40％PEG300 5％吐温80 80％盐水
溶解度：≥6.25 mg / mL（11.85 mM）; 澄清的溶液
2.逐一添加每种溶剂：10％DMSO 90％（盐水中20％SBE-β-CD）
溶解度：≥6.25 mg / mL（11.85 mM）; 澄清的溶液
3.每种溶剂一添加：10％DMSO 90％玉米油
溶解度：≥6.25 mg / mL（11.85 mM）; 澄清的溶液.
为了保证产品使用质量相关溶液，溶剂请找BIOFOUNT公司订购。
案例：
a) 3000 synchronized L1 larvae were transferred to seeded 6 cm NGM plates and immediately treated with hygromycin B. Images of control and treated plates were acquired 40 hours later. b) Survival assay on hygromycin B dilution. 3000 synchronised L1 larvae were plated on seeded 6 cm plates and immediately treated with hygromycin B at the specified final concentrations. The plates were scored for L4 larvae after 40 hours and for adults after 90 hours. The experiment was performed in triplicate error bars represent standard deviation. c) Selection of transgenic animals on hygromycin B. 10 synchronized L1 larvae carrying a hygromycin B resistance gene and expressing GFP/mCherry were mixed with the indicated number of wild type L1 larvae on a seeded 6 cm plate and immediately treated with hygromycin B (0.3 mg ml−1). The number of adult transgenic (fluorescence-positive) and non-transgenic (fluorescence-negative) animals was scored after 90 hours. The experiment was performed with two independent hygromycin B resistant transgenic strains.
